用泡沫分离法浓缩和分离蛋白质(Ⅰ)——设备及分离过程的研究

Concentration and separation ofproteins by foam fractionation(Ⅰ) Study on the apparatus and the separation process

提出一种新型泡沫分离设备即环流泡沫塔，以牛血清清蛋白（ＢＳＡ）的分离过程为例，对比了在环流泡沫塔与鼓泡塔中泡沫分离蛋白质的动力学行为，考察了操作参数（气速）、溶液体系的性质（ｐＨ和离子强度）对分离过程的影响。以表面张力作为描述蛋白质在水溶液中的表面活性的参量，研究了蛋白质溶液体系性质对其表面张力和泡沫分离效果的影响。结果表明，在接近蛋白质等电点处，溶液的表面张力最低，进行泡沫分离效果最佳。

A novel bubble column, namely loop flow bubble column, is developed for foam fractionation of protein from its aqueous solution. The effects of pH, ionic strength and gas flow rate on foam separation of bovine serum albumin (BSA) are investigated, respectively. The surface tension of BSA solution of different pH and ionic strength is measured to characterize the surface activity of the protein in aqueous solution. It is concluded that an optimal foam separation can be achieved at the pH close to the isoelectric point of the target protein, at which the surface tension of the protein solution approaches its minimum.