己烯雌酚荧光性质的研究及其分析应用

STUDY ON FLUORESCENT PROPERTY OF DIETHYLSTILBESTROL AND ITS APPLICATION

基于己烯雌酚与浓硫酸反应后的水解产物的荧光性质，建立了一种测量己烯雌酚的荧光光度分析方法．系统地研究了不同环境介质对产物荧光性质的影响，着重探讨了不同增敏剂在不同酸度下的作用机理．在比较了β－ＣＤ与ＣＴＭＡＢ的增敏作用之后指出：在强酸性条件下，β－ＣＤ的增敏效果优于ＣＴＭＡＢ；在强碱性条件下ＣＴＭＡＢ优于β－ＣＤ．并通过小分子醇对包络物荧光强度的影响对包络机理作了详细的阐述．利用本法测定片剂中己烯雌酚的含量，结果较好．

A new fluorimetric method of determination of diethylstilbestrol has been proposed and applied in the tablet. The effecting of pH on the fluorescence intensity has been discussed in details. We have also studied the reaction mechanism of the inclusion complexion of β Cyclodextrin and the effect of the surfactant cetyltrimethylammonium bromide (CTMAB)