血浆叶酸和同型半胱氨酸水平与结直肠癌的关系

The Relationship between Plasma Folate,Vitamin B_(12),Homocysteine Level and Colorectal Cancer

目的:研究结直肠癌患者血浆叶酸、同型半胱氨酸和维生素B12水平的变化,及其与肿瘤分期和p53基因表达的关系。方法:比较49例结直肠癌患者和51例非肿瘤患者血浆叶酸、同型半胱氨酸和维生素B12水平,并分析与肿瘤分期和癌组织p53基因表达的关系。结果:结直肠癌患者血浆叶酸和维生素B12水平显著低于非肿瘤对照者,分别为7.08±3.97ng·ml-1对11.07±5.60ng·ml-1和337.92±177.72pg·ml-1对479.67±219.43pg·ml-1(P均<0.001),血浆同型半胱氨酸水平则显著高于非肿瘤对照19.69±10.61μM对14.01±4.17μM(P=0.002),血浆叶酸、同型半胱氨酸和维生素B12水平与肿瘤分期和肿瘤组织p53蛋白表达无显著相关性。结论:叶酸缺乏是结直肠癌发病的危险因子。

Objective：To investigate plasma level of folate, homocysteine（Hcy） and vitamin B12 in relation to colorectal cancer risk and p53 expression. Methods：The plasma level of folate, Hcy and vitamin B12 of 49 patients with colorectal cancer（CRC） were measured and were compared with 51 gender and age matched subjects of no neoplasma. The plasma level of folate, Hcy and vitamin B12 were also compared with nuclear p53 accumulation examined by immunohistochemistry. Results Plasma level of folate and vitamin B12 were significantly decreased in CRC patients than those in controls. [7.08 ± 3.97 ng·ml^-1 vs. 11.07±5.60 ng·ml^-1 and 337.92＋ 177.72 pg·ml^-1 vs. 479.67±219.43 pg·ml^-1,respectively. （P〈0.001）]while plasma Hcy level increased in CRC patients than that in control subjects. [ 19.69 ＋ 10.61μM vs. 14.01±4. 17 μM（P = 0. 002）]. Plasma level of folate, homocysteine（Hcy） and vitamin B12 was not correlated with the TMN staging, or with the nuclear accumulation of p53. Conclusion： Folate deficiency is associated with the higher risk of CRC.