渗透处理三七细胞促使人参皂甙的有效释放

Effective Release of Ginseng Saponin in Cell Suspension Cultures of Panax Notoginseng Permeated with Dimethyl Sulfoxide

二甲基亚砜（ＤＭＳＯ）渗透处理三七悬浮培养细胞，使该细胞有效释放胞内皂甙。体积分数φ＝０．０２～０．０５的ＤＭＳＯ溶液短期处理三七细胞（３０ｍｉｎ），胞内皂甙释放量为３０～８０ｍｇ／Ｌ；φ＞０．１的ＤＭＳＯ溶液对细胞有严重伤害，胞内蛋白质大量放出。经φ＝０．０１的ＤＭＳＯ长期处理的三七细胞生长良好，且皂甙分泌量增加，培养基中可达１３０ｍｇ／Ｌ，约占皂甙总量的１０％。

Dimethy sulfoxide (DMSO) was used to permeate suspension cells of Panax notoginseng for effective release of a useful intracellular secondary metabolite ginseng saponin. When the cells were treated with DMSO of φ =0.02￣0.05 for 30 min, the saponin released into the medium was increased from 30 to 80 mg/L. At a DMSO concentration over φ =0.1, the cells were seriously damaged and a large amount of intracellular protein was also secreted. After the cells were treated with φ =0.01 DMSO for 5 successive subcultures, the ginseng saponin released was enhanced to the highest level of 130 mg/L in medium, which reached about 10% of its total amount.