食源性致病病毒基因芯片方法检测

Detecion of food borne vireses by gene-chip assay

目的将基因芯片技术用于食源性致病病毒诺如病毒、轮状病毒、甲肝病毒、星状病毒和脊髓灰质炎病毒的检测,达到同时检测2种以上病毒的需求。方法采用基因芯片方法检测贝类食品中引起人类腹泻的5种食源性致病病毒。结果所研制的检测5种病毒的基因芯片具有良好的特异性,在5种病毒之间无交叉反应;其灵敏度与荧光PCR方法基本一致;芯片在4℃条件下,保存7.5个月,性能稳定。结论建立了检测5种食源性病毒的基因芯片方法,同时该方法也可用于医疗检验目的。为基因芯片在微生物检测领域的应用提供基础依据。

Objective To establish the rapid, high - throughput method for food borne viruses detection and inspection. Methods Gene- chip was used to detect the food borne viruses which cause human diarrhea. Results The Gene - chip method was created to＇ detect 5 kinds of viruses from shellfish. The gene - chip method had the advantages of high specificity and the sensitivity the same as real time PCR. The stability of the Gene - chip was not changed in seven months under 4℃. Conclusion The method of Gene- chip has the priority of rapidity, high throughput and secsitivity in detecting food borne viruses, and also can be used on clinical purposes. This method provids the basic platform for further research in the field of detection of many kinds of viruses.