实时荧光定量PCR检测急性白血病的肝细胞生长因子mRNA的表达及其意义

The clinical significance of real-tlme fluorescence PCR quantification of hepatocyte growth factor mRNA expression in acute leukemia

目的定量检测急性白血病（AL）骨髓单个核细胞（MNCs）的肝细胞生长因子（HGF）mRNA的表达，并探讨其临床意义。方法制备67例初治AL患者的骨髓MNCs，提取定量MNCs的总RNA，并制备cDNA。应用实时荧光定量-PCR（FQ-PCR）检测HGF mRNA在AL的表达。结果HGF mRNA在AL组的表达显著高于对照组（6．936±1．613，0．407±0．170，P〈0．001），而急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）的表达差异无统计学意义（7．127±1．911，6．635±0．934，P〉0．05）。在AL中，M5呈显著强表达（9．998±1．454），高于M2、M3、M4、L1、L2和L3表达（P〈0．001）；而后者间表达差异无统计学意义（P〉0．05）。HGF mRNA在性别、年龄间的表达差异也无统计学意义（P〉0．05）。缓解组HGF mRNA的表达明显低于未缓解组（6．393±1．165，8．041±1．848，P〈0．005）。结论HGF mRNA在AL组与对照组、AL亚型间的表达存在差异，但在AML与ALL、AL患者的性别、年龄间的表达无差异。且低表达者疗效较好，提示HGF mRNA可能是AL诊治的又一理想指标。

Objective To detect quantitatively hepatocyte growth factor （HGF） mRNA expressions of bone marrow mononuclear cells （MNCs） in acute leukemia （AL） and investigate its clinical significance. Methods Total mRNA of quantitated bone marrow MNCs isolated from 67 de novo AL cases was extrated and then cDNA was synthesized. Expression of HGF mRNA was quantified absolutely using real-time fluorescence quantification PCR （FQ-PCR）. Results Expressions of HGF mRNA in a group of AL were higher significantly than these in a control group （6. 936 ± 1. 613, 0. 407 ± 0. 170, P 〈 0. 001 ）, but there was similarity between a group of acute myeloid leukemia （AML） and group of acute lymphoblastic leukemia （ALL） （7. 127 ± 1. 911, 6. 635 ± 0. 934, P 〉 0. 05 ）. In AL subtypes, the expression of M5 （9. 998 ± 1. 454） was higher than that of M2, M3, M4, L1, L2 and L3 （P 〈 0. 001 ） , but there ware no differences among the latters （P 〉 0.05 ）. Meanwhile, there was no statistical significance on the expressions of HGF mRNA between different age and sex （P 〉0. 05）. In addition, expressions of HGF mRNA in the remission group were lower than these in the non-remission group （ 6. 393 ± 1. 165, 8.041 ± 1. 848, P 〈 0. 005 ）. Conclusions There are statistical significances of the expressions of bone marrow MNCs HGF mRNA among the AL group and control group. As to AL subtypes, there are no statistically significant differences between AML and ALL as well as between different age and sex. Besides, lower HGF mRNA level is correlated with better curative effect. It is suggested that HGF mRNA is a suitable index for AL diagnosis and treatment.