鼠哺乳期二噁英暴露对子代牙槽骨生长发育的影响

Effects of lactational dioxin exposure to development of alveolar bone in SD rat offspring

目的研究鼠哺乳期二噁英类物质2，3，7，8-四氯二苯对二噁英暴露对大鼠仔鼠牙槽骨生长发育的影响。方法60天龄2，3，7，8-四氯二苯对二噁英暴露组和对照组仔大鼠被处死，将其带有磨牙的上颌骨行树脂包埋，制备硬组织磨片并在荧光显微镜下观察，对两实验组牙槽骨的组织形态、荧光标记情况及组织形态计量学参数进行比较。结果与对照组相比，二噁英暴露组牙槽骨骨小梁结构较为疏松，荧光标记较紊乱。实验组与对照组骨小梁宽度分别为（52．5±5．2）μm及（59．4±6．6）μm，二者差异有统计学意义（P〈0．05）；骨小梁数目分别为（3．27±0．23）mm^-1及（3．75±0．29）mm^-1，差异有统计学意义（P〈0．01）；骨小梁分离度分别为（217．3±37．6）μm及（177．6±33．8）μm，差异有统计学意义（P〈0．05）；矿化沉积率分别为（0．68±0．08）μm／d及（0．95±0．12）μm／d，差异亦有统计学意义（P〈0．05）。结论二噁英类物质哺乳期暴露显著降低了大鼠仔鼠牙槽骨的质、量，并影响其空间构型。

Objective To study the influence of lactational dioxin exposure （2, 3, 7, 8-tetrachlorodibenzo-p-dixon, TCDD ） on development of alveolar bone in SD rat offspring. Methods The rats of TCDD exposure group and control group were sacrificed and the alveolar bone with molars of PD60 rats in the two groups were embedded in resin. The sections were observed by fluorescent microscope. The alveolar bone formation was evaluated by histological examination, tetracycline fluorescence marker and quantitative histomorphometry. The indices of quantitative histomorphometry were compaired. Results The trabecular structure of alveolar bone was looser in TCDD exposure group than in the control group. The tetracycline fluorescence markers were more disorganized in TCDD group. The indices of quantitative histomorphometry of alveolar bone between two groups showed significantly different. Conclusions Lactational 2,3,7,8-TCDD exposure decreased the quality and quantity of alveolar bone in SD rat offspring. It is suggested that dioxins exposure may interrupt the spatial configuration.