气升式光生物反应器培养微藻溶氧和pH值变化规律研究

The change of dissolved oxygen and pH of the marine microalgae cultivation in air-lift photobioreactor

为了解决在光生物反应器养殖微藻过程中，溶解氧和pH值2个培养工艺参数的控制问题，分别在80L、350L和900L的3种规格气升式光生物反应器中培养湛江等鞭金藻（lsochrysis zhanjiangensis），高藻细胞浓度分别达到900×10^4，700×10^4，500×10^4 cell／mL，测定其中溶氧和pH值的日变化。结果显示，在光照度4000 lx以上时，湛江等鞭金藻光合作用强，表现为反应器中藻液溶氧较高，日最高溶氧分别可达17．91mg／L、15、84mg／L和12．7mg／L。所测定的藻液日pH值均在7．00～9．16变化。

To provide reference frame for efficient cultivation of microalgae in the air-lift photobioreator. The changes of dissolved oxygen （DO） and pH of 900 × 10^4 cell/mL, 700× 10^4 celt/mL and 500 × 10^4 celt/mL Isochrysis zhanjiangensis cultivation in 80 L, 350 L and 900 L photobioreators were investigated. The photosynthesis intensity of Isochrysis zhanjiangensis was high when the light strength was above 4 000 Ix. Under three densities, 900 × 10^4 cell/mL, 700 × 10^4 cell/mL, 500 × 10^4 cell/mL, the highest DO could reach 17.91 mg/L, 15.84 mg/L, 12.7 mg/L respectively; Compared with 80 L photobioreator, 350 L and 900 L high capability photobioreators were also good for the growth of Isochrysis zhanjiangensis. The variety of pH value in each photobioreators was in 7.00 -9.16 scope.