不同茶多酚中儿茶素的测定及柱层析法提取EGCG的比较

Determination of catechins in different tea polyphenols and comparison of EGCG isolation by column chromatography

应用高效液相色谱法对不同产地和不同纯度茶多酚中儿茶素的组成进行了研究,并考察了硅胶柱层析法对不同规格茶多酚中EGCG的分离纯化效果。实验表明,茶多酚中各儿茶素单体的含量不随茶多酚纯度的升高而增加,茶多酚中各儿茶素单体的相对含量与茶多酚的产地和生产工艺有着密切的关系。并且,针对茶多酚中不同儿茶素单体的分离纯化,应选择适当的茶多酚为原料。

The catechins composition of tea polyphenols with different origin areas and purities was investigated by HPLC, and the effect of silica gel column chromatography on the isolation of Epigallocatechin gallate （EGCG） from different tea polyphenols was compared. This experiment indicated that the mass of catechins in tea polyphenols did not increase with increasing purity of tea polyphenols. It was related to the origin area and techniques of tea polyphenols extraction. The right tea polyphenols must be chosen for the isolation of different catechins.