硫酸软骨素模板诱导片状纳米羟基磷灰石的仿生合成

Template Induced Biomimetic Synthesis of Nano Flake-like Hydroxyapatite by Chondroitin Sulfate

依据生物矿化的原理,以硫酸软骨素（ChS）为模板合成了片状纳米羟基磷灰石（HA）。用X射线衍射仪（XRD）、傅立叶变换红外光谱（FTIR）、透射电镜（TEM）和热重-差热分析仪（TG-DTA）对纳米HA的结构、组成、形貌和热性能进行表征。结果表明ChS的浓度很大程度地影响了HA的形貌,浓度较低时（0.1-0.3%质量分数）,形成短纤维状晶体;浓度较高时（≥0.5%质量分数）,则形成片状晶体。电导率测试结果表明,ChS和钙离子之间存在较强相互作用。本文还讨论了HA的生长机理。

Based on the principles of biomineralization, nano flake-like hydroxyapatite （HA） were synthesized through biomimetic method using Ca（NO3）2 · 4H2O and （NH4）3PO4 · 3H2O as reagents and chondroitin sulfate as template. The structure, chemical composition, morphology and thermal behavior of the samples were characterized by X-ray diffraction （XRD）, Fourier transform infrared spectroscope （FHR）, transmission electron microscope （TEM） and thermogravimetry-differential thermal analyzer （TG-DTA） respectively. The interaction between the polar groups of ChS and HA was investigated by electrical conductivity and UV-vis spectrum. The results show that the concentration of ChS significantly affects the morphologies of HA. Short fiber-like HA could be obtained at a low concentration of ChS. Flake-like hydroxyapatite particle can be synthesized using a relative high concentration （ ≥ 0.5wt% ） of ChS as template. The results of electricity conductivity indicate the interaction between ChS and calcium ions, which induced the nucleation and growth of HA on the molecule chain of ChS. The formation mechanism of templating growth HA by ChS was discussed.