海蛇乙醇浸出物对实验性大鼠关节炎的防治作用

Preventive and Therapeutic Effects of Alcohol Extracts of Bodies of Four Seasnake Species(AEBFSS)on Rat Experimental Arthritise

探讨大鼠灌胃给予海蛇乙醇浸出物（ＡＥＢＦＳＳ）对大鼠胶原性关节炎（ＣＩＡ）的预防和治疗作用。方法用自制的可溶性猪尾Ⅱ型胶原注入实验大鼠左后足造成胶原诱导性大鼠关节炎模型。结果大鼠在致炎前口服ＡＥＢＦＳＳ，当剂量达含４．０ｍｇ浸出蛋白／ｋｇ，可明显推迟ＣＩＡ发病时间，降低ＣＩＡ发病率和减轻关节炎症状；大鼠发生关节炎后口服ＡＥＢＦＳＳ，可明显抑制ＣＩＡ大鼠关节炎症的程度，其作用在用药后７天表现明显；关节组织病理检查表明，口服ＡＥＢＦＳＳ显著减轻了患鼠关节的炎症程度。初步分析表明，口服ＡＥＢＦＳＳ既降低了ＣＩＡ患鼠血清抗ＣⅡ抗体水平，又对患鼠耳迟发型超敏反应（ＤＴＨ）产生了明显的抑制作用。结论口服ＡＥＢＦＳＳ对大鼠胶原性关节炎有显著的防治作用，其机制同时涉及Ｔ和Ｂ淋巴细胞免疫。

bjective To investigate the preventive and therapeutic effects of oral AEBFSS on the rat Collagen-Induced Arthritis(CIA) Methods The rat CIA models were established by injecting the soluble Pig-Tail Collagen typeⅡ into the left legs of the rat Results The AEBFSS that had been taken orally before causing inflamation could significantly postpone coming on of the rat CIA,droped the incidence of the CIA,and alleviated the rat Arthritis,when the dose of the AEBFSS amounted to 4 0mg proteins/kg The AEBFSS taken after causing inflamation could ease the rat Arthritis,which would be manifested more evidently after administrating the AEBFSS for 7 days The pathological examinations of the rat joint revealed that the oral AEBFSS was able to significantly ease the CIA rat Arthritis too Analysis showed that the AEBFSS was not only able to reduce the serum anti-C Ⅱantibody, but also inhibit Delayed Type Hypersensitivity(DTH) of the ear in the rat with CIA Conclusions The oral AEBFSS has significant effects of prevention and curing on the rat CIA,the mechanism of which involves T and B lymphocyte immunity simutaneously