摘要
巢蛋白(Nestin)属于中等纤维基因家族,在增殖较快的神经前体细胞中表达。该基因被克隆后,作为神经前体细胞的标记基因得到广泛应用。本文中,我们根据小鼠巢蛋白cDNA序列,设计了一对引物,在确定了反转录PCR反应的最佳反应条件后,详细地考察小鼠巢蛋白mRNA在中枢神经系统发育过程中的表达规律,发现巢蛋白mRNA在小鼠第10天胚胎的脑中表达,到第14天表达量达到最高,后下降;在出生后的小脑中,表达也有类似规律,出生后第5天,表达量最高,其后下降。在成年小鼠各组织中,均未检测到巢蛋白mRNA的表达。
Intermediate filament Nestin is a marker of neural precursor cells. Its expression pattern during mouse brain development was studied in the present investigation. A pair of primers based on the sequence of mouse Nestin were found best to be suit for the reverse transcriptional PCR (RT-PCR) with this pair of Nestin Primer. Using RT-PCR, Nestin transcripts were detected during the development of the central nervous system (CNS) of mouse, from embryonic day 10 to adult brain. We found that the Nestin showed a transient expression in the development of cerebrum. At the embryonic day 14, the expression of Nestin mRNA reached the highest peak, and was then down-regulated. In the postnatal cerebellum, Nestin gene was also expressed in a transient pattern. The highest peak of Nestin mRNA expression emerged at postnatal day 5, and was then down-regulated. Nestin mRNA expression in 10 kinds of adult mouse tissues did not yield any positive results.
出处
《生理学报》
CAS
CSCD
北大核心
1997年第6期657-665,共9页
Acta Physiologica Sinica
基金
国家自然科学基金!39470245