影响病毒免疫治疗肿瘤疗效诸多因素的实验研究

STUDY ON THE VIRAL IMMUNOLOGY TREATMENT TO TUMOR MOUSE

甲型流感病毒ＰＲ８株两次注射治疗Ｓ１８０腹水瘤小鼠（６周龄），第一次注射后小鼠存活率为５２％，间隔不同时间二次注射ＰＲ８，小鼠存活率均有不同程度提高，达６０％—９０％；小鼠植入Ｓ１８０细胞后第２、５、７、１０天分别给予病毒治疗，其存活率分别是７０％、３５％、１７．５％和０，对照组则１００％死亡。为观察荷瘤鼠体内抗病毒抗体对疗效的影响，首先用ＰＲ８免疫小鼠，待产生抗体后植入Ｓ１８０细胞再行病毒治疗，小鼠存活率为６２．５％；而未经病毒免疫过的荷瘤鼠，其存活率仅３３．３％。ＰＲ８治疗“老”龄荷瘤鼠（１０月龄），存活率为０，平均生存时间为２２．３天，对照组为２０．５天。环磷酰胺可增加病毒对瘤细胞的感染率，二者协同作用，能提高小鼠存活率。

Tumor mice were injected the influenza A virus PR8 strain twice with the result of 52% cure rate for the first time and 60% to 90% for second at different intervals. The tumor mice were implanted the s180 eclls and then carried on the viral treatment at different time(2,5,7and 10 days).The respective cure rates were 70%,30%,17.5% and 0%.However,all the control groups died from the tumor.The tumor mice were immunized with the PR8 first,then the virus treatment was carried on after the antibody produced.The experiment group showed the cure rate of 62.5%,while the contral group was 33.3%.When senite mcie were treated with PR8 strain ,the curerate was 0, and the average surrival time was 22.3% days,the contral group was 20.5% days. Cyctophos-phamide could improve the infection rate of the virus to the tumor cell from 60% to 80%.