鼻咽癌细菌L型感染与P^(53)基因突变的研究

BACTERIAL l-FORM INFECTION AND P53 GENE MUTATION IN NASOPHARYNGEAL CARCINOMA

用免疫组化和革兰染色等技术，对６９例鼻咽癌进行细菌Ｌ型和突变型Ｐ５３蛋白检测，并以２０例鼻咽粘膜慢性炎作为对照。结果发现，革兰染色鼻咽癌细菌Ｌ型检出率为７９．７％，与免疫组化Ｌ型抗原表达阳性率（６９．６％）具有一致性（Ｐ＞０．０５）。鼻咽癌Ｐ５３蛋白表达阳性率为６５．２％，与粘膜慢性炎的Ｐ５３蛋白表达（１／２０）有显著性差异（Ｐ＜０．００５），且Ｌ型阳性病人的Ｐ５３蛋白表达阳性率（７８．２％）明显高于Ｌ型阴性病人（１４．３％），两者有显著性差异（Ｐ＜０．００５）。结果表明，细菌Ｌ型感染与突变型Ｐ５３蛋白过度表达存在着明显相关性。提示，细菌Ｌ型感染参与了Ｐ５３抑癌基因的失活，基因突变可能是细菌Ｌ型致鼻咽癌作用的机制之一。

69 cases nasopharyngeal carcinoma (NPC)and 20 cases chronic nasopharyngitis (NPS)associated with bacterial L-form were examined by Gram's staining and immunohistochemical. The result showed that the positive rate of the infection of bacterial L-forms was 79.7% in NPC group with Gram's staining, and the antigen of staphylococcus aureus L-forms in 69.6% cases has been detected hy immunohistochemical technique There were not differences between was the results obtained in both staining methods (P>0.05).The positive rate of mutant P53 protein (MP53) in NPC patients were 65.2% respectively,the expression of MP53 were significantly higher in NPC group than in that NPS group (P<0.005).In NPC group, the positive rate of the expression of MP53 in bacterial L-form positive patients (78.2%) were significantly higher than those in bacterial L-form negitive patients (P<0.005).The results indicate that there in corrlation between bacterial L-form infection and MP53 expression, and that P53 gene mutation may be related with bacterial L-form infection.Bacterial L-form infetion may be one of the cause of nasopharyncarcinogenesis mechanisms through mutation of P53 gene.