用RT-PCR和RFLP对禽传染性支气管炎病毒中国分离株的分型

DIFFERENTIATION OF AVIAN INFECTIOUS BRONCHITIS VIRUS ISOLATED IN CHINA USING POLYMERASE CHAIN REACTION AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS

用ＲＴ－ＰＣＲ方法获取了禽传染性支气管炎病毒（ＩＢＶ）标准毒株Ｍ４１、Ｈ５２、Ａ５９６８和国内分离株Ｄ４１、Ｆ、Ｇ的Ｓ１基因，对它们做ＲＦＬＰ分析，发现Ｄ４１、Ｆ株属于马萨诸塞血清型、Ｇ株为变异株。对用ＲＴ－ＰＣＲ和ＲＦＬＰ来区分ＩＢＶ的分型方法的应用理论和前景进行了论述。

Using RT-PCR technique, we got S1 genes of IBV reference strains M41、H52、A5968 and strains D41、F、G isolated in China. From the RFLP patterns, using the restriction enzyme Hae Ⅲ as was used for PCR products of these strains, we found that strain D41 and strain F belong to Massachusette serotype, and strain G is a variant. This paper also discussed the scientific basis and the application of RT-PCR/RFLP analysis of IBV S1 gene to IB epidemiological detection and control.