蓖麻蚕血细胞系的建立及同源核型多角体病毒的体外复制

ESTABLISHMENT OF TWO BLOOD CELL LINES FROM THE PHILOSAMIA CYNTHIA RICINI DONOVAN AND IN VITRO REPLICATION OF ITS NUCLEAR POLYHEDROSIS VIRUS

蓖麻蚕血细胞系的建立及同源核型多角体病毒的体外复制沈中建陈梅琴吴祥甫（复旦大学生命科学学院病毒研究室，上海２００４３３）关键词蓖麻蚕，细胞系，核型多角体病毒，体外复制细胞培养技术已成为昆虫病毒基础和应用研究中极其有用的手段。尤其在人们利用杆状病毒作...

Two blood cell lines (Pcr-S1 and Pcr-S2)from the Philosamia cynthia ricini Donovan were established.Mostof the cells were suspended in the medium,except that as mall number of cells attached themselves to the glass.Round cells of about 9.2-23.5μ in diameter were predominant in Pcr-S1 cell population,but in Pcr-S2 cell population,the most predomin ant were the spherical cells of about 20μ in diamater.The population doubling times of Pcr-S1 and Pcr-S2 were51.6h and 60.2 h,respectively. Although these two cell lines had been derived from the same insect species,they varied largely in their response to challenge with the Pcr NPV.The percentage of infected cells with polyhedra in Pcr-S1 cell line was 52.9% and each infected cell produced 21 polyhedra on average.This demonstrated that Pcr-S1 cell line could be an excellent in vitro system for studing Pcr NPV replication and for biotechnical application as well.