逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究

Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes

目的：检测比较蚊体内的黄病毒。方法：采用RT－PCR技术及常规的C6／36细胞培养结合免疫荧光法。结果：无论实验感染的登革热病毒蚊虫标本，还是从野外捕捉的标本，均表明RT－PCR检出黄病毒的阳性率比常规法高，且快速、敏捷，操作简便；常规法检出周期长（2～3周），操作复杂、繁琐和反复多次，检出率低。但常规法能通过细胞病变，观察病毒的毒力，这是RT－PCR所不及的。结论：以RT－PCR结合常规法进行虫媒病毒的研究，将更有利于探索蚊虫与虫媒病毒的关系和内在规律。

We have made a comparison for detecting Flaviviruses in mosquitoes between reverse transcription-polymerase chain reaction (RT-PCR) and C6/36 cell culture-McAb-IFA (conventional method). The results showed that the pasitive rates of flavivirus infection by RT-PCRwhether Dengue viruses infected mosquitoes in laboratory or naturally catched mosquitoes werehighter than that by conventional method. The duration of detected Flavivirus infection was shorterby RT-PCR than by conventional method and the operation was simpler. But toxicity of flaviviruscould be observed through cells pathological changes for the latter and it not for the former.