2006年北京市延庆县布鲁杆菌病高暴露人群血清学监测结果分析

Analysis on serology of brucellosis in high exposure population in Yanqing County of Beijing in 2006

目的了解北京市延庆县人间及畜间布鲁杆菌病（简称布病）感染现状，为有效地控制布病在人间的发病与流行提供科学依据。方法2006年在延庆县采取整群抽样方法，以高暴露人群和养殖的奶牛作为观察对象。使用酶联免疫吸附试验（ELISA）和试管凝集试验（SAT）方法进行高暴露人群血清抗体检测，并对其基本情况、职业、疫苗接种史、临床表现等进行流行病学调查。同时采集喂服S2布鲁杆菌减毒活疫苗1个月后的奶牛血清．用SAT法检测布鲁杆菌抗体水平。结果共采集216名奶牛养殖人员血液样本，其中27例抗体滴度呈阳性，阳性率为12．50％，经调查均无临床症状；抽取2185头奶牛血样做抗体检测，1488头奶牛血清抗体阳性．阳性率为68．10％，所有奶牛均无显性感染。结论延庆县奶牛养殖人员中布病隐性感染率较高，今后需加强从业人员在工作中的自身防护，积极开展相关的宣传教育工作，并坚持开展畜间检疫、免疫、淘汰病畜及人群血清学监测工作．以保障布病高暴露人群的健康。

Objective To understand current status of brucellosis infection in both human and animal in Yanqing County to get solid scientific basis for controlling the disease and to prevent the spread of epidemic. Methods Cluster sampling method was used in this survey in stock raisers and ELISA and SAT were performed to inspect serologic antibody of Brucella. At the same time an epidemic investigation was carried out, including the basic personal situation, occupational history, vaccination history, clinical symptom, and so on, Resutis In a total of 216 cow raisers whose blood was detected, 27 cases showed positive in titer test of Brucella antibody with a ratio of 12.50%. However, all the antibody carriers did not have any clinical symptoms. In all 2185 cows that were collected for antibody test, 1488 cases had positive serological antibody with a ratio of 68.10%. And all the positive cows also did not have any clinical symptoms. Conclusions The subclinical infection of brucellosis is rather high in Yanqing County＇s stock raisers. It is self-protection of the stock raisers, epidemic knowledge propaganda, serological surveillance in human and animal, immunize of healthy cows, and eliminate of diseased cows that are urgent in order to keep the public in health.