黑龙江省肾综合征出血热患者血清汉坦病毒致病株分析

Analysis on Hantaan virus in hemorrhagic fever patients with renal syndrome in Heilongliang

目的对黑龙江省肾综合征出血热（HFRS）患者血清中汉坦病毒（HV）进行分离、扩增，寻找其与国际标准株（76-118株）之间的差异。方法应用反转录-巢式-聚合酶链反应（RT-nested-PCR），对50例发病不同时期的HFRS患者血清中HV进行分离、扩增，并将扩增产物进行序列测定及同源性分析。结果发病7d内患者血清HV检出率为36.36％（8／22），发病8～14d的患者血清HV检出率为13．04％（3／23），发病15d以上的5例患者均未检出。选取的7例样本（第1、9、18、31、37、38、44号标本）HVS基因片段序列与76-118株S基因片段的同源性分别为90．24％、86．72％、89．97％、89．16％、86．45％、87．26％和89．43％。结论发病7d内患者检出率明显高于其他时期；黑龙江省HV致病株与国际标准株存在一定差异，说明HV除具有宿主依赖性外，还具有地域的影响。

Objective To separate and amplify Hantaan virus（HV） in serum of hemorrhagic fever patients with renal syndrome（HFRS） in Heilongjiang, and look for its difference from international standard type strain（76-118 strains）. Methods HVs of different phase in the serum of 50 HFRS patients were separated and amplified by RT- nested-PCR, its products were analyzed the amplified by sequencing. Results Detectable rate of HV in the patients serum was 36.36%（8/22） in 7 days after onset, it was 13.04%（3/23） in patients having an onset 8 days to 14 days earlier, 5 cases were not detectable 15 days after onset. Comparing the sequence of HV S gene fragment, sample 1,9,18,31,37,38,44 strain had a homology of 90.24% ,86.72% ,89.97% ,89.16% ,86.45% ,87.26% and 89.43% to 76-118 strains, respectively. Conclusions The positive rate is the highset in 7 days after onset. Nucleotide sequence difference exists between pathogenic strain of Heilongjiang＇s HV and international standard strain, indicating that not only hosts but also locations can affect HV.