摘要
目的:探讨Galectin-9基因和蛋白在结直肠癌中的表达及其临床意义。方法:运用荧光实时定量PCR及免疫组织化学染色方法检测45例手术切除的结直肠癌标本和20例正常结直肠上皮组织中Galectin-9基因和蛋白的表达,分析Galec-tin-9的表达与结直肠癌临床病理特征的关系。结果:Galectin-9基因在结直肠癌组织中表达低于正常结直肠上皮组织(P<0.05),Galectin-9蛋白在结直肠癌组织中表达阳性率为62%,在正常结直肠上皮组织中表达阳性率为90%,差异具有统计学意义(P<0.05)。Galectin-9的低表达与结直肠癌的远处转移相关(P<0.05),与患者的年龄、性别、肿瘤浸润深度、淋巴结转移、肿瘤分化程度、肿瘤分期及肿瘤部位无显著相关。结论:Galectin-9在结直肠癌中低表达,并且与肿瘤的远处转移相关,有可能成为结直肠癌判断预后的标志物以及基因治疗的潜在靶点。
Objective ：To study the expression of Galectin-9 mRNA and protein in colorectal cancer （CRC） and its clinical significance, nethods：Galectin-9 mRNA and protein levels were detected in 45 cases of CRC and 20 cases of normal colorectal epithelial tissues by real-time PCR analysis and immunohistochemisty staining. The relationship between the expression levels of Galectin-9 and clinicopathological features was analyzed. Results ：The levels of Galectin-9 mRNA were lower in CRC than that in normal colorectal epithelial tissues （ 19.80 ± 17.37 vs 45.34±43.81, P 〈0.05 ）. The positive expression ratios of Galectin-9 protein in CRC and normal colorectal epithelial tissue were 62% （ 28/45 ） and 90% （ 18/20 ）, respectively; the difference was of statistical significance （ P 〈 0.05 ）. The low expression of Galectin-9 correlated with the distant metastasis of CRC （P 〈 0.05 ）. But it had no correlation with the age and sex of patients, depth of tumor invasion, lymph node metastasis, differentiation degree, tumor stage, and tumor location. Conclusion：The expression of Galectin-9 mRNA and protein in CRC is weak. Weak expression of Galectin-9 is associated with distant metastasis of CRC. Galectin-9 may become a prognostic factor or a gene therapy target for CRC.
出处
《肿瘤》
CAS
CSCD
北大核心
2008年第5期419-422,共4页
Tumor
基金
加拿大Terry Fox慈善基金资助.
