缺血性脑卒中ALOX5AP基因SG13S114T/A和SG13S89G/A多态性研究

To study relationship between polymorphism of SG13S114T/A and SG13S89G/A in the ALOX5AP gene and thrombotic stroke

目的探讨5-脂氧合酶激活蛋白基因的SG13S114T/A和SG13S89G/A的多态性与缺血性脑卒中的关系及性别差异。方法对确诊的100例缺血性脑卒中和100例对照者从外周静脉血提取DNA,经聚合酶链反应-单核苷酸多态性方法(PCR-PLFP)检测SG13S114T/A和SG13S89G/A位点酶切多态性。结果(1)ALOX5AP的SG13S114A等位基因频率在缺血性脑卒中男性患者的出现率(35.7%)明显高于对照组(28.4%);(2)SG13S114AA明显增加男性缺血性脑卒中的发病风险1.59倍;(3)SNPSG13S89G/A与缺血性脑卒中的易感性不相关。结论ALOX5AP基因SG13S114的AA基因型和A等位基因频率增加了本地区男性缺血性脑卒中的发病风险。

Objective The study is to assess whether genetic variants in the ALOXSAP encoding SG13Sll4T/A and SG13S89G/A will influence the risk for stroke and diffirence of sexual. Methods Totally 100 patients with thrombotic stroke who were admitted in our department and 100 control,polmorphisms of SG13S114T/A and SG13S89G/A in the ALOX5AP were genetyped by the polymenase chain reaction and the restriction enzyme analysis. The multivariate logistic regression model was used to exclude the influence of the conventional vascular risk factors on stroke. SPSS12.0 software was conduct for analysis. Results （ 1 ） The frequence of SD13Sll4A allcel in the ALOX5AP was significantly higher in male patients with thrombotic stroke （35.7%） than in controls （28.4%）. （2） The SG13S114AA genetype was significantly associated with a 1.59 - fold risk for thrombotic stroke in men. （ 3 ） The SG13S89G/A variant was not associated with stroke. Conclusion A common genetic variant SG13Sll4T/A in the ALOX5AP gene is associated with an increased risk for atherothrombotic stroke in Ningxia male.