呼吸道合胞病毒感染细胞超微结构的改变及清肺口服液含药血清对其作用的研究

Ultrastructure Influence of Serum Contained Qing Fei Oral Liquid in Cell Infected with Respiratory Syncyntial Virus

目的：观察呼吸道合胞病毒（RSV）感染细胞超微结构改变及清肺口服液含药血清对其的影响。方法：细胞分为正常细胞组、病毒攻击组、空白血清组、感染后和感染时含药血清组，病毒攻击后3～5h，利用电镜观察各组人胚肺成纤维细胞超微结构的变化。结果：病毒感染组，细胞核增大，核质比增加，核仁凝聚，核膜凹陷成夹层，包涵体形成，线粒体凝聚，粗面内质网扩张，溶酶体增多，核染色质浓缩，细胞外可见病毒颗粒。含药血清感染时给药组，细胞核大小基本正常，有的轻度增大，无核仁凝聚，无包涵体形成，粗面内质网形态正常，线粒体基本正常，偶有凝聚现象，细胞间很少找到病毒颗粒。含药血清组感染后给药组，细胞核增大，核仁凝聚，粗面内质网扩张，线粒体凝聚，但程度较轻。结论：RSV感染人胚肺成纤维细胞后，细胞可出现超微结构的改变，清肺口服液含药血清可以减轻这种改变，感染时给药比感染后给药作用更明显。

Objective： To observe the uhrastructure of respiratory syncyntial virus （RSV） - infected CCC - HPF - 1 cell and the effect of serum - contained Qing Fei Oral Liquid. Methods： Changes in CCC - HPF - 1 cell were studied using electron microscopy 3～5h after cell exposed to RSV. Serum contained medicine were added at the same time or after RSV infected. Results and Conclusion ： The noticeable alteration were observed in virus group, the alteration was alleviated after serum - contained Qing Fei Oral Liquid were added, especially at the same time with RSV infected.