艾灸对老年人衰老症状及外周血单个核细胞细胞周期的影响

Effects of Moxibustion on Senescent Symptoms and Cell Cycle in PBMC in Aged People

目的:探讨艾灸延缓衰老的作用及对细胞周期的影响。方法:将61名60～75岁老年人随机分为艾灸治疗组和衰老对照组,并另设正常青年人对照组。艾灸组以温和灸肾俞和关元穴,疗程3个月。采用积分表法观察艾灸对老年人衰老症状的变化情况,用流式细胞术检测老年人外周血单核细胞细胞周期变化。结果:艾灸组较衰老对照组衰老症状积分明显降低(P<0.01),综合疗效、主症有效率均明显升高(P<0.01);G0/G1期细胞比例明显减少(P<0.01),PI指数显著升高(P<0.01)。结论:提示艾灸不仅可明显改善老年人衰老症状,且可能通过降低细胞周期G0/G1期细胞比例,升高PI指数,增加有丝分裂原的反应能力和合成DNA的能力,使细胞进入S期,从而发挥延缓细胞衰老的作用。

Objective： To investigate the effect of moxibustion on delaying senescence and influence of ceil cycle. Methods ： Selected 61 aged people during 60 -75 years old were randomly divided into two groups including the moxlbustion group and the aged control group. Selected young people were used as the normal control group. Warming moxibustion on points Shenshu （BL23） and Guanyuan （CV4） were applied to the treated group under treatment for 3 successive months. The effects of moxibustion on aging symptom of aged people were observed with integral table. The expressions of ceil cycle in peripheral blood mononuclear ceil （PBMC） of aged people were measured using flow cytometry. Results ： Compared with the aged control group, the moxibustion groups have significant differences in the scores of senescent symptoms and therapeutic effects of comprehend and main symptoms （ P 〈0.01）, the ceil percentage at G0/G1 phase in PBMC of aged people is decreasd and the index number of PI is increased significantly（ P 〈0.01 ）. Conclusion： This suggests that moxibustion not only markedly improve senescent symptoms but also probably reduce the ceil percentage at G0/G1 phase, increase the index number of PI, increase the reaction ability of mitogen and the ability of synthetic DNA to make ceil into S phase and to delay ceil senescence.