实时定量PCR测定大肠癌细胞系中ERCC1mRNA水平及草酸铂药物抵抗的关联性分析

Study on relationship between oxaliplatin antitumor resistance and ERCC1mRNA level in colorectal carcinoma cell lines

目的分析草酸铂处理前后ERCC1 mRNA水平的变化,探讨肠癌细胞中ERCC1表达与草酸铂药物抵抗性的关联。方法以标准化肠癌细胞为研究模型,SRB细胞抑制率实验评价不同肠癌细胞对草酸铂的敏感性,实时定量PCR测定草酸铂处理前后的变化。结果不同肠癌细胞对草酸铂的敏感性不同。草酸铂处理前后ERCC1 mRNA水平变化不明显。无论草酸铂处理前后,IC50与ERCC1 mRNA水平呈正相关。结论肠癌细胞对草酸铂的敏感性与肠癌细胞中固有的ERCC1表达水平密切相关,可能作为预测草酸铂使用有效性与安全性的分子标志物。

Objective To analyze ERCC1 mRNA level before and after oxaliplatin treatment, and find the re lationsbip between oxaliplatin anti-tumor resistance and ERCC1 mRNA level. Methods Standard colorecta carcinoma cell lines to oxaliplatin sensitivity were measured by SRB assay, and ERCC1 mRNA levels in al cell lines were measured by real-time PCR. Results Sensitivities to oxaliplatin in different cell lines were dif ferent. There was a positive relationship between IC40 and ERCClmRNA in all cell lines whether before o after oxaliplatin treatment. Conclusions Sensitivities to oxaliplatin are closely related to inherent ERCC1 mRNA levels in colorectal cell line; ERCC1 is an important molecular marker to predict validity and securi ty of oxaliplatin treatment.