血管紧张素-2对血管内皮细胞[Ca^(2+)]i的影响及合贝爽的保护作用

Effects of angiotensin-2 on concentration of intracellular free calcium in vascular endothelial cells and protection of herbesser

目的:观察血管紧张素-2(Ang-2)对血管内皮细胞[Ca2+]i的影响及合贝爽的保护作用。方法:将血管内皮细胞分为空白对照组、Ang-2组、Ang-2+合贝爽组。Ang-2组加入10-7mol/L Ang-2,Ang-2组+合贝爽组加入10-7mol/LAng-2和1 mg/L合贝爽。各组在药物作用60 min后,用激光共聚焦显微镜测量各组细胞的[Ca2+]i。结果:①Ang-2组的细胞内[Ca2+]i显著高于空白对照组(P<0.01);②Ang-2+合贝爽组的细胞内[Ca2+]i显著低于Ang-2组(P<0.01)。结论:Ang-2可引起细胞内[Ca2+]i的显著增加,而合贝爽对Ang-2所致的[Ca2+]i增加具有保护作用。

Objective： To explore the effects of angiotensin - 2 （Ang - 2） on concentration of intracellular free calcium in vascular endothelial cells（VEC） and protection of herbesser. Methods：Cultured VEC were divided into control group, Ang - 2 group and Ang - 2 ＋herbesser group. 10^-7mol/L Ang - 2 was added into the culture solution of Ang - 2 group, and 10^-7mol/L Ang - 2 and 1 mg/L herbesser were added into the culture solution of Ang - 2 ＋ herbesser group. After the cultured VEC were treated for 60 min, the concentration of intracellular free calcium of three groups was measured by laser scanning confocal microscopy. Results：The concentration of intracellular free calcium of Ang - 2 group was significantly higher than that of control group （P〈0. 01）, the concentration of intracellular free calcium of Ang - 2＋herbesser group was significantly lower than that of Ang - 2 group（P〈0. 01）. Conclusion： Ang -2 can induce elevation of concentration of the intraeellular free calcium in VEC and herbesser can inhibit the induction of Ang - 2.