日本血吸虫32kDa重组抗原的进一步研究

Further Studies on 32kDa Recombinant Antigen of Schistosoma japonicum

用慢性日本血吸虫病病人血清筛选以βgill构建的日本血吸虫中国大陆株成虫cDNA表达库，获得一阳性克隆Sjgt4，将其插入片段经聚合酶链反应（PCR）法扩增后亚克隆入表达载体pTrcHisB并进行表达，获37kDa融合蛋白，它可溶于8M尿素，并可被日本血吸虫病病人血清中特异抗体所识别。以此37kDa融合蛋白免疫家免所得的抗血清，可特异地识别日本血吸虫成虫抗原的67kDa分子。

A gene fragment encoding 32kDa protein in a positive clone Sjgt4 was selected from adult S.japonicum λ gtll cDNA expression library with the pooled serum from the chronic patients infected with S.japonicum and sub-cloned into expression carrier pTrcHis B.Thus,the expressed fusion protein 37kDa was obtained It was soluble in 8M urea and could be recognized by serum from rabbits or human infected with S.japonicum.The anti -serum from the rabbits immunized with fusion protein 37kDa could specifically recognize 67kDa protein of adult worm antigens of S.japonicum.