温阳活血方上调慢性马兜铃酸肾病大鼠肾组织血管生成素1 mRNA表达

Wenyang Huoxue Recipe up-regulates the expression of angiopoietin-1 mRNA in rats with chronic aristolochic acid nephropathy

目的:观察温阳活血方对慢性马兜铃酸肾病大鼠肾组织血管生成素(angiopoietin,Ang)表达的影响,并探讨其保护作用机制。方法:将28只雄性SD大鼠随机分为正常对照组、模型组和温阳活血方组。模型组大鼠予关木通水煎液10ml/(kg.d)灌胃,温阳活血方组于模型组基础上,再予温阳活血方30g/(kg.d)灌胃。20周后,采用实时逆转录聚合酶链式反应方法检测3组大鼠肾组织中Ang-l和Ang-2mRNA的表达。结果:与正常对照组比较,模型组大鼠肾组织Ang-1mRNA表达明显减少(P<0.01),Ang-2mRNA表达明显升高(P<0.01);与模型组大鼠比较,温阳活血方组大鼠肾组织Ang-1mRNA表达升高(P<0.01),Ang-2mRNA表达下降,但差异无统计学意义(P>0.05);肾脏病理显示温阳活血方组肾损害较模型组明显减轻。结论:温阳活血方通过上调Ang-1mRNA的表达发挥对慢性马兜铃酸肾病大鼠肾脏保护作用。

Objective：To investigate the effects of Wenyang Huoxue Recipe（WRHXR）,a compound traditional Chinese herbal medicine for warming yang and promoting blood flow,on the expression of angiopoietin mRNA in rats with chronic aristolochic acid nephropathy induced by Caulis Aristolochia Manshuriensis（CAM） decoction,and to explore the protection mechanism of WYHXR against kidney damage.Methods：Twenty-eight male SD rats were randomly divided into normal control group,CAM group and WYHXR-treated group.Rats in the normal control group（n=8） and CAM group（n=10） were intragastrically administered with normal saline 10 ml/（kg·d） or CAM decoction 10 ml/（kg·d） respectively.Rats in the WYHXR-treated group（n=10） were intragastrically administered with WYHXR 30 g/（kg·d） and CAM decoction 10 ml/（kg·d）.The expressions of Ang-l and Ang-2 mRNAs were detected by real-time reverse transcription polymerase chain reaction（RT-PCR） after 20-week treatment.Results：Compared with the normal control group,the expression of Ang-l mRNA was significantly decreased,and the expression of Ang-2 mRNA was significantly increased in the CAM group（P〈0.01）.Compared with the CAM group,the expression of Ang-l mRNA was increased in the WYHXR-treated group（P〈0.01）.The expression of Ang-2 mRNA had no significant difference between the CAM group and the WYHXR-treated group（P〉0.05）.Renal pathology showed that renal damage in WYHXR-treated group was significantly reduced as compared with the CAM group.Conclusion：WYHXR can up-regulate the expression of Ang-l mRNA,which may be its action mechanism in protecting the kidneys.