核糖体DNA内转录间隔区序列标记在寄生虫学中的应用

Application of Ribosomal DNA Internal Transcribed Spacer in Parasites

目的:介绍核糖体DNA(rDNA)的内转录间隔区(ITS)序列的结构特征和在寄生虫学研究应用中的最新研究进展。方法:检索有关文献进行综合分析。结果:rDNA ITS1和ITS2序列为寄生虫的鉴定提供了非常有价值的遗传标记。结论:核糖体DNA上的ITS区域序列的进化速度较其它区域快,具有高变异性,可以从中获得大量的遗传信息,已成为在种和亚种水平上对寄生虫进行分类鉴定的有效手段。

Objective： To introduce the recent progress on the structural features of Ribosomal DNA internal transcribed spacer sequence and application in parasitology. Methods： Relative literatures were summarized and analyzed. Results： Ribosomal DNA internal transcribed spacer land internal transcribed spacer 2 sequence provided very valuable genetic markers in identification of parasites. Conclusion：Ribosomal DNA internal transcribed spacer evolutionary faster than other regions. It had high variability and a lot of genetic information could be acquired. So Ribosomal DNA internal transcribed spacer has become a effective means in the classification identification in parasites species and subspecies.