不同人群幽门螺杆菌表型分型研究

Typing of H. pylori Strains Isolated from Patients with Gastric Diseases

作者报道了从各型胃病患者胃粘膜中分离的112株幽门螺杆菌，用聚丙烯酰胺凝胶电泳（SDS－PAGE）及Westernblots分析，可将HP菌分为Ⅰ-Ⅳ型，而其中Ⅳ型又分为Ⅳ-1至Ⅳ-5五个亚型；不同HP间存在明显的蛋白抗原差异和LPS／LOS的表达差异；为今后开展HP疫苗研究提供了重要线索。

Helicobacter pylori, an organism recently described in association with gastritis and pepticlesions. The degree of relationship between these micro - organisms was studied on molecular level usingSDS- PAGE protein profiles and immunoblot patterns. Study on a total of 112 lysates of H. pylorishowed some differences in protein patterns of different H. pylori strains. However, the difference becamemore apparent when studied by immunoblot analysis. According to SDS - PAGE protein, strains isolatedfrom gastric mucous of the patients were identified as types Ⅰ-Ⅳ, while type Ⅳ can be further classifiedas sub-type Ⅳ -Ⅰto Ⅳ-5 analysis of SDS - PAGE protein patterns proved to be an excellent method ofcharacterizing those H. pylori, different from traditional methods.