PCR方法捕获环境水样中嗜肺军团菌DNA

The Detection of Legionella Pneumophila DNA with Polymerase Chain Reaction in Water Samples

嗜肺军团菌是军团病的主要病原体，它所导致的军团病病例占临床病例的80％以上，多数军团病暴发由嗜肺军团菌所引起。通过针对嗜肺军团菌种保守基因（mipgene）设计的引物，我们建立了一种多聚酶链反应（PCR）检测方法，可以准确捕获环境标本中的嗜肺军团菌，同时具有很好的特异性，其最低可检出18cfu／ml的细菌。该方法的建立为嗜肺军团菌病的诊断以及在军团病流行病学调查中嗜肺军团菌病原的追踪提供了一条便捷、准确、可靠的途径。

Legionella pneumophila has been known as an important pathogen causing LegionnairesDisease (LD), responsible for more than 80 per cent of clinical LD cases and majority of the LDoutbreaks. In order to detect the existence of Legionella pneumophila strains in water samples, we havedeveloped a ploymerase chain reaction method, using two primers synthesized according to the reportedLegionella pneumophila mip gene nucleotide sequence. This method was proved to be highly sensitive(18 cfu/ml) and specific. Two water samples were detected positive. This newly developed methodprovided a quick, accurate way not only to detect Legionella pneumophila in the diagnosis of LD but alsoin the capture of suspected pathogen during epidemiologic investgation when LD outbreaks.