肺癌中Etsl mRNA和c-Met蛋白表达细胞芯片的研究

Research about cell microarray of Etsl mRNA and e-Met protein expression in lung cancer

目的进行细胞芯片的研制，为快速、规范、简便、经济地进行各种细胞学检查提供一种新方法。方法应用自制的细胞芯片检测装置（专利申请号：2005200278140），制成7×16点阵细胞芯片。对切片进行HE染色、免疫组织化学染色和荧光原位杂交技术检测。结果制成的细胞芯片为112点阵，HE染色检测结果与相应涂片结果完全一致；应用细胞芯片进行了Etsl mRNA荧光原位杂交和c-Met免疫组化染色，所得阳性结果与各例相应涂片检测结果均一致；将细胞芯片检测结果与进行EtslmRNA荧光原位杂交和c-Met免疫组化染色的Ⅲ＋Ⅳ期肺癌的组织芯片阳性率进行比较，差异均无统计学意义（P〉0．05）。结论细胞芯片操作简单，节约试剂，减少实验误差，具有诊断快速准确、特异性高、信息量大的特点，在临床诊断、科研和流行病学筛查中将有广泛的应用前景。

Objective To develop cell microarray and apply a rapid,normative,simple and economical method for the scientific research in cell fields and clinical work. Methods An 7 × 16 cell microarray was designed using the home-made cell microarray survey setting （the patent appling No. 200520027814 0）. HE stain,immunol histochemistry,fluorescence in situ hibridization. Results The made-up cell microarray was 112 points, and the diagnosis resulted from the cell microarray was totally same to that from the corresponding spread pieces under the microscope. After FISH stain of Etsl mRNA and IHC of c-Met,the positive expression was totally same to that from the routine spread pieces. Comparing the positive expression rate of Etsl mRNA and IHC of c-Met in the Ⅲ＋Ⅳ stages of lung cancer by the cell microarray to that by the tissue microarray, there was no significant difference between them （ P 〉0.05）. Conclusions Cell microarray is a simple,economical method and has low rate of experimental error. It has advantages of rapid diagnosis, high specificity, and large information. It will be broadly applied in clinical diagnosis, scientific research and epidemiologic screening.