摘要
目的探讨P38的核转位与DTT诱导的食管癌细胞凋亡的关系。方法采用DTT处理食管癌Eca109细胞,未加药组作为对照。流式细胞仪技术检测细胞凋亡率;免疫组化方法检测P38的磷酸化水平及磷酸化P38的核转位情况。结果与对照组相比,DTT处理组细胞凋亡率明显升高。免疫组化结果显示:处理组P38的磷酸化水平明显高于对照组(P<0.01),且处理组p-P38大部分定位于核内。结论p-P38核转位参与了DTT诱导的食管癌Eca109细胞凋亡。
Objective To study the relationship between nuclear translocation of p-P38 (phosphorylated P38) and apoptosis induced by DTT. Methods Esophageal carcinoma cell line Ecal09 was treated with DTT. The untreated cells served as the control group. The esophageal carcinoma cell apoptosis was detected by flow cytometry. The P38 phosphorylation was detected by immunohistochmestry. Results The apoptotic rate of DTT treated group was higher than that of control group(P 〈0.01 ). The phosphorylation level of the treatment group was much higher than that of the untreated group (P 〈0.01 ). DTT treatment induced nuclear translocation of p-P38. Conclusion The nuclear translocation of p-P38 may be associated with esophageal carcinoma cell apoptosis induced by DTT.
出处
《河南职工医学院学报》
2008年第2期107-109,共3页
Journal of Henan Medical College For Staff and Workers