主要细胞周期蛋白在人类正常胃肠道黏膜组织中的表达规律

The laws of major Cyclins expressed in human normal gastrointestinal mucous tissue

目的研究人类主要细胞周期蛋白(Cyclin D1、E、A、B1)在正常胃肠道黏膜组织中的表达规律。方法收集手术切除正常胃肠道黏膜组织标本共33例,应用间接免疫荧光双标法在激光扫描共聚焦显微镜下检测Cyclins蛋白的表达及其差异;24例正常大小肠黏膜组织应用免疫组织化学研究人类主要细胞周期蛋白(Cyclin D1、E、A、B1)在正常胃肠黏膜组织中的表达规律。结果①在正常胃肠道黏膜组织中的Cyclins表达以Cyclin A、B1为主,Cyclin D1,Cyclin E低表达或无表达,除了Cyclin D1和Cyclin E组间无统计学意义外,其余各组方差分析均有统计学意义,P<0.01;免疫组织化学染色结果和免疫荧光染色结果相符合;②胃和肠黏膜间4种Cyclins的表达,Cyclin B1有显著性差异(P<0.01),其它Cyclins(A、D1、E)的表达率无显著性差异(P>0.05)。结论在人类正常胃肠道黏膜组织中的Cyclins表达以Cyclin A、B1为主,胃肠道黏膜组织中Cyclin B1表达有差异,大肠黏膜中Cyclin B1的表达显著高于胃组织中Cyclin B1的表达。

Objective to investigate the laws of Cyclins （Cyclin D1 ,E ,A, B1 ） expression in the normal gastrointestinal mucous tissue. Methods The expression and differential expression of Cyclins protein in 33 cases of normal gastrointestinal mucous tissue from collection of operation were detected by indirect immunofiuorescence double-staining methods under laser scanning confocal microscope method and using immunohistochemistry method to study the laws of Cyclins （D1 ,E,A,B1） expression in the normal small, large intestines. The total numbers of the tissue samples were 24. Results ① The expression of Cyclins in the normal gastrointestinal mucous tissue mainly were the Cyclin A, B1. And Cyclin D1 , Cyclin E were at a low level of expression or no obvious expression was detected. Each group had a statistic significance except between Cyclin D1 and Cyclin E groups, there was no statistic significance, P 〈 O. O1 ;the resuits of immunohistochemistry and immunofiuorescence dyes were similar. ② Among of the four types of Cyclins expression rate, in the stomach and large intestines, only Cyclin B1 had a predominant difference（P 〈0. O1 ）. Others Cyclins （A,D1,E） in the stomach and large intestines, there was no statistic significance （P 〉 O. 05）. Conclusion Cyclins expression in normal gastrointestinal mucous tissue is mainly Cyclin A, B1. Expression of Cyclin B1 in the gastrointestinal mucous tissues is with obvious difference. Expression of Cyclin B1 in the intestinal mucosa is higher than that in the gastric mucosa.