吖啶红-共振荧光光谱法测定水溶液中的苯酚

Resonance Fluorescence Spectra of Acridine Red and Its Analytical Application

在硫酸介质中吖啶红的分子共振荧光信号强，发射峰尖而对称，受干扰因素少。基于苯酚能抑制KBrO，氧化吖啶红的反应，建立了阻抑动力学共振荧光分析法测定痕量苯酚。方法的线性范围为8．5×10^-4-1．00mg／L苯酚，检出限0．26μg／L。本方法用于环境水样中苯酚的测定，RSD小于2．94％，样品加标回收率为97．5％～102．5％。

The properties of resonance fluorescence of Acridine Red （AR） were studied. AR itself has a strong resonance fluorescence signal that the resonance fluorescence peak is tine and symmetry in sulphuric acid medium, with foreign substances don＇t interfere with it. Based on the inhibitory effect of phenol on the oxidation of AR by potassium bromate, a inhibitory resonance fluorescence method was proposed for the determination of trace phenol. The linear range was 8. 50 x 10^-4 - 1. 00 mg/L with the detection limit of 0.26 μg/L. This method is simple, sensitive, rapid and can be applied to the determination of trace phenol in water samples of environment satisfactorily. The RSD was less than 2. 947% and the recoveries range were 97.5% - 102.5%.