摘要
目的:探讨Bio-oss胶原与骨髓基质细胞(bone marrow stromal cells,BMSCs)的生物相容性,为用组织工程技术修复牙周缺损提供理论依据。方法:体外培养恒河猴BMSCs,将BMSCs与Bio-oss胶原复合进行体外培养。用激光扫描共聚焦显微镜检测细胞与材料的黏附,MTT法检测细胞的增殖,并行碱性磷酸酶(ALP)活性测定。采用SAS6.12软件包对所得数据进行t检验。结果:BMSCs能黏附于Bio-oss胶原上;MTT结果显示,在2d、5d时,对照组和实验组间无显著性差异(P>0.05),8d时,两组间有显著性差异(P<0.05);在各时间点,对照组和实验组间ALP活性均无显著性差异(P>0.05)。结论:Bio-oss胶原与恒河猴BMSCs有良好的生物相容性,可作为组织工程材料应用于牙周骨缺损的修复。
PURPOSE： To study the biocompatibility of Bio-oss collagen with cultured bone marrow stromal cells. METHODS： The bone marrow stromal cells （BMSCs） of rhesus monkey were cultured with Bio-oss collagen in vitro. Cell attachment was observed with laser confocal microscope. Cell proliferation rates were assessed with MTY assay. ALP activity was also detected. The data was statistically analyzed with SAS6.12 software package for Student＇s t test. RESULTS： The rhesus BMSCs could attach to the surface of Bio-oss collagen. In cell proliferation rates, there was no significant difference between the control group and experimental group at 2-day and 5-day（P〉0.05）. However, at 8-day, the difference was significant （P〈0.05）. For ALP activity, there was no significant difference among different time point （P〉 0.05）. CONCLUSIONS： The Bio-oss collagen has good biocompatibility with rhesus BMSCs, and could be used to repair periodontal bone defects as a biomaterial in tissue engineering.Supported by National ＂863＂ Project （Grant No. 2002AA205013） and Shanghai Municipal Education Development Fund （Grant No.2002-02）.
出处
《上海口腔医学》
CAS
CSCD
2008年第2期161-164,共4页
Shanghai Journal of Stomatology
基金
国家“863”计划资助课题(2002AA205013)
上海市教育发展基金资助课题(2002-02)
