分子标记技术及其在大豆疫霉根腐病研究中的应用

Molecular Marker Techniques and Their Application in the Studies of Soybean Phytophthora Root Rot Disease

随着遗传学的不断发展,遗传标记的种类和数量也不断增加。与其他标记技术相比,分子标记技术具有许多明显的优越性,因而应用也日趋广泛。综述了RFLP、RAPD、SSR、AFLP、SCAR、SNP、SRAP等几种主要DNA分子标记技术原理和方法及其在大豆疫霉根腐病研究中的应用。

With the continuously development of genetics, the types and quantities of genetic marker techniques also increased rapidly. Compared with other marker techniques, molecular marker techniques had been applied widely for their superiority. This paper summarized the principle and method of several leading molecular marker techniques such as restriction fragment length polymorphism（RFLP）, random amplified polymorphic DNA（RAPD）, simple sequence repeats（SSR）, amplified fragments length polymorphism（AFLP）, sequence characterized amplified region（SCAR）,single nucleotide polymorphism（SNP） and sequence related amplified polymorphism（SRAP） and their application in the studiesof soybean phytophthora root rot disease.