甲状腺肿瘤3号染色体短臂杂合性缺失的研究

Loss of heterozygosity on chromosome 3p in thyroid tumors

目的 探讨甲状腺肿瘤中3号染色体短臂（3p）杂合性缺失（LOH）状态及其临床意义。方法 收集74例甲状腺肿瘤标本，包括20例甲状腺腺瘤（FA）、24例滤泡性甲状腺癌（FTC）和30例乳头状甲状腺癌（PTC）。通过PCR扩增和银染分析其3p上11个微卫星位点的杂合性缺失状态。结果 FIE的LOH频率达到71％（17／24），PTC中30％（9／30），FA中10％（2／20）。FTC的3p LOH频率显著高于FA和PTC（P〈0．01）。FTC中存在两个最小共同缺失区，分别位于3p26-pter和3p14．2-3p22。PTC上存在一个最小共同缺失区，位于3p25．2-26．1。结论 FTC的3p LOH频率显著高于FA和PTC。3p的3个最小缺失区上可能存在着与FTC和PTC发生发展相关的肿瘤抑制基因。

Objective To study the loss of heterozygosity （LOH） on chromosome 3p in thyroid tumors. Methods LOH at 11 microsatellite loci was analyzed in 74 cases of thyroid tumors （ including 20 follicular adenomas, 24 follicular thyroid carcinomas and 30 papillary thyroid carcinomas） by polymerase chain reaction and silver stain. Results LOH on chromosome 3p was detected in 71% of follicular thyroid carcinoma （ 17/24）, 30% of the papillary thyroid carcinoma （9/30） and 10% of the follicular adenoma （2/20） case. Two minimal common deleted regions （CDR） （3p26-pter and 3p14.2-3p22） involving significant sites of LOH has identified in follicular thyroid carcinoma. There was also one CDR （ 3p25. 2- 26. 1 ） in papillary thyroid carcinoma. Condnsions LOH is more frequently identified in follicular thyroid carcinoma than in papillary thyroid carcinoma and follicular adenoma. The 3 CDR on chromosome 3p may harbor tumor suppressor genes involved in the pathogenesis of follicular thyroid carcinoma and papillary thyroid carcinoma.