摘要
目的 探讨甲状腺肿瘤中3号染色体短臂(3p)杂合性缺失(LOH)状态及其临床意义。方法 收集74例甲状腺肿瘤标本,包括20例甲状腺腺瘤(FA)、24例滤泡性甲状腺癌(FTC)和30例乳头状甲状腺癌(PTC)。通过PCR扩增和银染分析其3p上11个微卫星位点的杂合性缺失状态。结果 FIE的LOH频率达到71%(17/24),PTC中30%(9/30),FA中10%(2/20)。FTC的3p LOH频率显著高于FA和PTC(P〈0.01)。FTC中存在两个最小共同缺失区,分别位于3p26-pter和3p14.2-3p22。PTC上存在一个最小共同缺失区,位于3p25.2-26.1。结论 FTC的3p LOH频率显著高于FA和PTC。3p的3个最小缺失区上可能存在着与FTC和PTC发生发展相关的肿瘤抑制基因。
Objective To study the loss of heterozygosity (LOH) on chromosome 3p in thyroid tumors. Methods LOH at 11 microsatellite loci was analyzed in 74 cases of thyroid tumors ( including 20 follicular adenomas, 24 follicular thyroid carcinomas and 30 papillary thyroid carcinomas) by polymerase chain reaction and silver stain. Results LOH on chromosome 3p was detected in 71% of follicular thyroid carcinoma ( 17/24), 30% of the papillary thyroid carcinoma (9/30) and 10% of the follicular adenoma (2/20) case. Two minimal common deleted regions (CDR) (3p26-pter and 3p14.2-3p22) involving significant sites of LOH has identified in follicular thyroid carcinoma. There was also one CDR ( 3p25. 2- 26. 1 ) in papillary thyroid carcinoma. Condnsions LOH is more frequently identified in follicular thyroid carcinoma than in papillary thyroid carcinoma and follicular adenoma. The 3 CDR on chromosome 3p may harbor tumor suppressor genes involved in the pathogenesis of follicular thyroid carcinoma and papillary thyroid carcinoma.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2008年第5期305-308,共4页
Chinese Journal of Pathology
基金
浙江省医药卫生科学研究基金[2007(B)218]
关键词
甲状腺肿瘤
染色体
人
3对
杂合子丢失
Thyroid carcinoma
Chromosomes,human,pair 3
Loss ofheterozygosity