鸡apoA5基因单核苷酸多态性及其与屠体性状的关联研究

Polymorphisms of chicken apoA5 gene and association with carcass traits of chickens

以丝羽乌骨鸡和隐性白洛克正反交产生的F2代为实验群体,采用PCR-SSCP和DNA测序的方法检测鸡载脂蛋白A5(apoA5)基因的单核苷酸多态性(SNPs),并将所发现的SNPs与体重、胸肌重、腿肌重、心脏重、肝脏重和腹脂重等屠体性状进行关联分析。结果发现,鸡apoA5基因5′-调控区C-169T,外显子2C600T、T635C,外显子3C841G、C914T、C1142G、C1394T共7个突变位点。其中外显子2突变位点C600T、T635C对12周龄腹脂重、腹脂率、肝脏重和心脏重有显著影响(P<0.05),根据PCR-SSCP的结果将其分为6种基因型(AA,AB,AC,BB,BC,CC):其中CC型个体的腹脂重和腹脂率显著高于AA型、AB型、AC型、BB型、BC型(P<0.05);AC型个体的肝脏重显著低于AA型、AB型、BB型、BC型和CC型的肝脏重(P<0.05);BC型个体的心脏重显著低于BB型的心脏重(P<0.05)。

Seven single nucleotide polymorphisms（SNPs） were identified by PCR-SSCP and sequencing in the chicken apoA5 gene in F2 chickens from an experimental cross of White Plymouth Rock × Silkies. One SNP（C-169T） located on the 5′-regulatory region, another two in the second exon were transitions of C to T（600） and T to C（635）. Four SNPs in the third exon were found, which were C841C~ C914T, C1142G; C1394T. The association of the polymorphisms with carcass traits was investigated. The most significant results were yielded from primer apoA3F/R： the abdominal fat weight of CC chickens were significantly higher than that of AA, AB, AC, BB and BC chickens（P〈0.05）; AC chickens had lower liver weight than that ofAA, AB, BB, BC and CC （P〈0.05）; BC chickens had lower heart weight than that of BB（P〈O.05）.