鲤鱼微卫星标记与体重、体长和体高性状的相关分析

Correlation analysis of microsatellite DNA markers with body weight,length and height of common carp(Cyprinus carpio L.)

利用47个鲤鱼微卫星标记,对柏氏鲤和荷包红鲤抗寒品系自交F2代的92个个体基因组DNA进行检测,得到162个等位基因,各座位等位基因数2～6个,片段长度100～444bp,有效等位基因数1.3069～4.2288,杂合度0.2361～0.7677,位点多态信息含量0.5368。利用统计软件SPSS的GLM程序对47个微卫星标记与鲤鱼体重、体长和体高相关性进行了分析,结果表明HLJ695、HLJ716、HLJ739、HLJ759、HLJ774、K16与体重、体长和体高相关,HLJ776与体高相关。对同一标记不同基因型间进行多重比较,找到与3种性状相关的基因型。

Forty-seven microsatellite markers were selected to analyze the genomic DNA of 92 progenies derived from the recombinant inbred lines （RIL） of common carp, which came from the cross between Barbless carp and Hebao-cold tolerance red carp. The results showed that a total of 162 different alleles were found, and the number of alleles in each locus was 2 to 6. The DNA fragment length was 100 bp to 444 bp, and the number of mean valid alleles was 1.3069 to 4.2288. The value of heterozygosity was 0.2361 to 0.7677, and the mean polymorphism information content （PIC） was 0.5368. A GLM procedure was used to analyze the effects of these 47 microsatellites on body weight, length and height. Results uncovered HLJ695, HLJ716, HLJ739, HLJ759, HLJ774 and 1（16 had a significant impact on body weight, length and height, and HLJ776 had a significant impact on height. In addition, the genotypes of these correlative loci were determined.