CYP1B1基因缺失小鼠眼前房角组织结构的观察

Structural observation of the anterior chamber angle in CYPIBI-nuil mice

目的 研究细胞色素P450181（CYPlBl）基因对小鼠眼前房角组织结构的影响。方法 实验研究。采用成年CYP1B1基因缺失小鼠模型，以同龄C57BL／6J小鼠为对照。通过塑料包埋切片技术，用光学显微镜和透射电子显微镜观察两组小鼠眼前房角组织的形态和超微结构。结果 C57BL／6J小鼠眼前房角组织发育正常；CYP1B1基因缺失小鼠存在不同程度的房水引流系统发育畸形，表现为小梁网发育不良，网眼狭窄，小梁细胞结构变异，有时可见小梁柱间有异常存在的均质膜，Sehlemm管狭窄或缺失，虹膜突从虹膜根直达全部小梁网。这些异常改变在CYP1B1基因缺失小鼠眼前房角内呈局灶性分布。结论 CYP1B1基因在房角的发育过程中有重要作用，其缺失可导致小梁网、Schlemm管、虹膜突等房水滤过道的结构异常，可能影响房水引流系统的代谢和功能。

Objective To determine the effects of CYP1B1 gene on the structure of anterior chamber angle in mice. Methods It is experimental study. Adult CYP1B1-null mice were used, in comparison C.57BL/6J mice was used as control. The mice eyes were enucleated and plastic-embedded and the anterior chamber angle were sagittally sectioned in 3μm thickness. The morphology and structure of the eye tissues was studied by light microscopy and transmission electron microscopy. Results The anterior chamber angle in C.57BL/6J mice were well developed. But in CYPIBl-null mice, a variety of anomalies were found in the these tissues, including hypoplastie and compressed trabecular meshwork, abnormal structure of trabecular cells, a basal lamina extending from cornea over the meshwork, small or absent Sehlemm＇s canal, and long iris process extending from the iris root to the trabeeular meshwork. These pathological changes was demonstrated focally and the rest of the angle in CYP1B1-null were normal. Conclusions CYP1B1 gene plays an important role in the development of the anterior chamber angle. Deletion of this gene leads to the abnormalities in trabecular meshwork, Sehlemm＇s canal and iris process, which may have some influence on the metabolism and function of the outflow facilities.