p38在大鼠骨性关节炎模型中的表达

Expression of p38 in the chondrocytes of rat osteoarthritis mode

目的检测大鼠骨性关节炎(OA)软骨细胞中丝裂素活化蛋白激酶(MAPK)p38的表达。方法取大鼠20只,雌雄各半,以任一后腿膝关节作为实验组(OA)组,另一侧为手术对照(C)组。OA组离断大鼠前交叉韧带及切除内外侧半月板前半部分建立大鼠骨性关节炎模型;C组仅打开关节腔,缝合切口。饲养8周后摄X线片后取关节软骨观察,通过HE、甲苯胺蓝(PG)观察软骨形态学的改变,免疫组织化学染色及免疫印迹(Western blot)检测磷酸化p38(P-p38)。结果与C组相比,OA组膝关节软骨表面粗糙,软骨被破坏;C组关节表面光滑。免疫组织化学染色及Western blot检测OA组膝关节P-p38阳性细胞表达明显增多。结论大鼠骨性关节炎模型软骨细胞中p38 MAPK被激活。p38 MAPK被激活可能是造成骨性关节炎的又一环节。

Objective To detect mitogen-activated protein kinase p38 in the chondrocytes of rat osteoarthritis model. Methods The osteoarthritis model was established by cutting off anterior cruciate ligament and excising the front part of interior and lateral meniscus on one hind leg in 20 rats （group OA）,the other hind leg was operated on for opening the articular cavity only as control（group C）.Rontgen ray photographs of two joints were taken after breeded for 8 weeks and the articular cartilages were taken for observing the morphological changes by HE and toluidine blue O,and detecting P-p38 expression through immunohistochemical study and Western blot.Results In contrast to group C,the surfaces of the articular cartilages in group OA were rough and destroyed. Immunohistochemical studies and Western blot conformed that P-p38 expression in group OA was much more than that in group C. Conclusion p38 MAPK is activated in the chondrocytes of rat osteoarthritis model,which may be another way to result in osteoarthritis.