重组人血管内皮抑素联合顺铂治疗鼠B16恶性黑色素瘤移植模型的实验研究

Effects of recombinant human endostatin combined of cisplatin on the growth of xenografled malignant melanoma in nude mouse

目的探讨重组人内皮抑素(恩度)联合顺铂化疗对恶性黑色素瘤的作用。方法建立B16恶性黑色素瘤的裸鼠移植模型20只,随机分为恩度组、顺铂组、恩度联合顺铂组(联合组)和对照组。治疗后处死裸鼠,计算肿瘤体积抑瘤率,用免疫组化法测微血管密度(MVD)及瘤体血管内皮生长因子(VEGF)的表达。结果恩度组、顺铂组和恩度联合顺铂组(联合组)的肿瘤抑制率分别为17.52%、45.72%和59.40%,联合组的肿瘤生长明显受到抑制(P<0.05)。免疫组化结果显示,联合组MVD低于其他组(P<0.05);恩度组、顺铂组和联合组的VEGF表达均明显低于对照组(P<0.05)。结论恩度联合顺铂有协同抗肿瘤作用,能有效抑制恶性黑色素瘤的生长。

Objective To study the effect of recombinant human endostatin（YH-16,endostar）combined with cisplatin（DDP）on the growth of xenografted malignant melanoma in nude mouse.Methods After the establishment of the model,mice were divided into 4 groups with five mice each.The drug was given intraperitoneally for consecutive 10 or 14 days：PBS （0.2ml/d for 1 to 14 days d1-14）,DDP （2mg·kg^-1·d^-1,for 1 to 10 days）,YH-16 （1.5mg·kg^-1·d^-1,for 1 to 14 days）,and DDP ＋ YH-16. Tumor volume was measured on the 21st day.The expression of vascular endothelial growth factor（VEGF） was examined.The tumor inhibition rate （TIR） was calculated.Results TIR of YH-16 group,DDP group and YH-16 ＋DDP group was 17.52%,45.72% and 59.40%,respectively.Tumor growing of YH-16 ＋DDP group was inhibited significantly （P〈0.05）.Microvascular dentity（MVD） of YH-16 ＋DDP group was also less than that of the other groups（P〈0.05）.The expression of VEGF in YH-16 group,DDP group and YH-16＋DDP group was remarkably degressed（P〈0.05）.Conclusion YH-16 in combination with DDP has synergistic effect on inhibitory activity against the growing of malignant melanoma.