多形性腺瘤及恶变组织中bal-2表达

Expression of bcl2 in Benign and Malignant Pleomorphic Adenoma of Salivary Glands

采用免疫组化方法，观察ｂｃｌ－２（Ｂｃｅｌｌｙｍｐｈｏｍａ－２）基因产物在多形性腺瘤及其恶变组织中的表达与分布，以图像分析仪进行计数分析。结果显示肿瘤组织中以导管样上皮细胞、粘液样细胞以及软骨样细胞的阳性表达较高，而鳞状上皮团块中，ｂｃｌ－２表达极低。各组阳性病例比例：对照组５例慢性诞腺炎为８０％（４／５），１５例多形性腺瘤为８０％（１３／１５），７例多形性腺瘤恶性病变为８６％（６／７）。图像分析仪计数阳性细胞显示慢性诞腺炎为５％，多形性腺瘤为４２％，而恶性病变为５７％，统计与分析表明良性及恶性多形性腺瘤与对照组比较，差异有显著性（Ｐ＜０．０５），而良性和恶性组间，差异无显著性（Ｐ＞０．０５）。

bcl2 is a apoptosissuppressing oncogene,and plays an important role in oncogenesis.bcl2 protein was detected by immunohistochemistry in 5 cases with chronic sialadenitis,15 cases (benign) and 7 cases (malignant) of pleomorphic adenoma of salivary glands.The results showed that bel2 expression in 4/5 cases with chronic sialadenitis,13/15 cases with benign and 6/7 cases with malignant pleomorphic adenoma were observed,respectively.Image analysis indicated 5%,42%,and 57% of total cells were positive for bcl2 in three groups,respectively.These findings suggest bcl2 gene may play an important role in developmen of pleomorphic adenomas.