日本血吸虫线粒体nad4基因部分序列的多态性研究

Polymorphism in the Partial Mitochondrial nad4 Gene of Schistosoma japonicum

采用PCR技术及DNA序列分析技术对采自湖南省4个不同地区的日本血吸虫的线粒体NADH脱氢酶基因亚基Ⅳ(nad4)部分片段(pnad4)进行克隆及序列分析,获得480bp的pnad4序列。与GenBank上的日本血吸虫相应基因序列进行比对,发现pnad4序列有一定的种内差异(0.4%～1.3%)。

The partial NADH dehydrogenase subunit 4 （pnad4） fragment was amplified by polymerase chain reaction （PCR） from Schistosoma japonicum collected from 4 areas in Hunan province, and the amplicons were cloned and sequenced. A 480 bp sequence was obtained and the nucleotide difference between samples from the four locations were 0.4%-1.3%. Low level of sequence variability between and within different populations of S.japonicum was detected. The results are worthwhile for further study on the population genetic structures of S.japonicum.