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FTA/PCR检测加入饮料中的隐孢子虫 被引量：2

FTA/PCR Detection for Cryptosporidium Added in Beverage
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摘要 用建立的FTA/PCR方法检测饮料样品中隐孢子虫,可检测到隐孢子虫卵囊1.0个/ml,且在6h内完成。该方法敏感性较高,省时,是检测饮料样品中隐孢子虫的较好方法。 An established FTA/PCR protocol was applied to detect Cryptosporidium oocysts which were added in beverage, and proved to be as sensitive as 1.0 oocyst/ml detected within 6 hours. The study indicates that this technique is sensitive, time-saving and easy to perform.
作者 李晓虹 闫东丽
机构地区 上海出入境检验检疫局 华东理工大学
出处 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2008年第2期158-159,共2页 Chinese Journal of Parasitology and Parasitic Diseases
基金 上海市科委技术标准专项资助项目(No.05dz05008)~~
关键词 隐孢子虫 FTA/PCR 检测 Cryptosporidium FTNPCR Detection
分类号 R382.3 [医药卫生—医学寄生虫学]
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参考文献8

  • 1陈佩惠.人体寄生虫学[M].第4版.北京:人民卫生出版社,1996.73-77.
  • 2张龙现,蒋金书,刘群,宁长申,朱引洁,吴绍强.巢式PCR检测隐孢子虫卵囊的研究[J].畜牧兽医学报,2003,34(5):509-514. 被引量：20
  • 3Xiao L, Escalante L, Yang C, et al. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus[J]. Appl Environ Microbiol, 1999, 65 (4): 1578-1583.
  • 4US Environmental Protection Agency. Method 1623: Cryptosporidium and Giardia in water by filtration, immunomagnetic separation, and fluorescent antibody[R]. Office of Water, Washington, DC Publication, 1999b. EPA-821-R-99-006.
  • 5Orlandi PA, Lampel KA. Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa[J]. Clin Microbiol, 2000, 38(6): 2271-2277.
  • 6Orlandi PA, Frazar C, Carter L, et al. Detection of Cyclospora and Cryptosporidium [A]. From Fresh Production,Isolation and Identification by Polymerase Chain Reaction (PCR) and Microscopic Analysis[C]. Chapter 19A (revised), Bacteriological Analytical Manual Online, 2004.1-9.
  • 7Geoff H, Jacqueline MH, Robert M. A rapid and simple method of detection of Blephaisma japonicum using PCR and immobilisation on FTA paper[J]. BMC Ecology, 2003, 3: 7.
  • 8Xiao LH, Fayer R, Ryan U, et al. Cryptosporidium taxonomy: recent advances and implications for public health[J]. Clin Microbiol reviews, 2004, 17 (1) : 72-97.

二级参考文献15

  • 1马良,陈雅棠,刘约翰.聚合酶链反应检测粪便中微小隐孢子虫[J].中国寄生虫学与寄生虫病杂志,1996,14(2):111-114. 被引量：35
  • 2Gasser R B. PCR-based technology in veterinary parasitology [ J ]. Vet Parasitol, 1999,84 : 229- 258.
  • 3Morgan U M, Thompson R C A. PCR detection of cryptosporidium: the way forward? Parasitol Today, 1998,14 : 241 -- 244.
  • 4Clark D P. New insights into human cryptosporidiosis[J].Clin Microbiol Rev, 1999,12(4):554--563.
  • 5Leng X, Mosier D A. Oberst. Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces[ J ]. Appl Environ Microbiol, 1996,62 : 643-647.
  • 6Xiao L, Escalante L, Yang C F, et al. Phylogenetic analysis of Cryptosporidium parasites based on the small subunit ribosomal RNA gene locus[J]. Appl Environ Microbiol, 1999,65 : 1578-- 1583.
  • 7Morgan U M, Pallant L, Dwyer B W, et al. Comparison of PCR and microecopy for detection of Cryptosporidium in human fecal samples: clinical trial [ J ]. J Clin Microbiol,1998,36:995--998.
  • 8MacKenzie W R,ScheU R,Blair K A,et al. Massive outbreak of waterborne Cryptosporidium infection in Milwaukee,Wisconsin:recurrence of illness and risk of secondary transmission[J]. Clin Infect Dis, 1995,21 : 57-62.
  • 9Sulaiman I M, Xiao L, Lal A A. Evaluation of Cryptosporidium parvum genotyping techniques[J ]. Appl Environ Microbiol, 1999,65:4431-4435.
  • 10芦圣栋.现代分子生物学实验技术(第2版)[M].北京：中国协和医科大学出版社,1999.61-149.

共引文献19

同被引文献26

引证文献2

二级引证文献2

中国寄生虫学与寄生虫病杂志
2008年 第2期

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