肝癌组织中p33^(ING1b)表达水平及其与p53关系和临床意义

Expression of p33^(ING1b) and its Relationship with the Expression of p53 in Hepatocellular Carcinoma

目的探讨肝癌组织中p33ING1b表达水平及其与p53的关系和临床意义。方法用逆转录聚合酶链反应(RT-PCR)检测55例肝癌及其癌旁组织p33ING1b mRNA水平,同时用免疫组化法检测p53蛋白表达。结果肝癌组织p33ING1b mRNA表达量为(0.410±0.175),明显低于癌旁组织(0.529±0.203)(P<0.05)。p33ING1b表达与肝癌的病理分级、淋巴结转移、包膜浸润相关,而与年龄、性别、肿瘤大小、癌栓形成、合并肝硬化无关。p53蛋白阳性肝癌组p33ING1b表达(0.492±0.196)明显高于p53蛋白阴性组(0.363±0.176)(P<0.05)。结论p33ING1b表达下调在肝癌发生、发展中起重要作用,可以作为肝癌分化、侵袭性及预后的评价指标。p33ING1b表达异常可能是野生型p53在肝癌细胞的失活机制之一。

Objective To investigate the p33^ING1bmRNA expression level and its relationship with p53 in hepatocellular carcinoma（HCC） and to evaluate its clinical significance. Methods Reverse transcriptase-polymerase chain reaction （RT-PCR） method was used to detect the p33^ING1b mRNA level in tissues from liver cancer and the adjacent lesion. The expression of p53 protein was also evaluated with method. Results The expression of p33m^ING1b mRNA in cancer tissues （0. 410 ±0. 175 ） was significantly lower than that in adjacent tissues of cancer（ 0. 529 ± 0. 203） （ P 〈 0.05 ）. The expression of p33^ING1b Was significantly correlated with the pathological grades ,lymph node metastasis and capsular infiltration,but not with sex,age,tumor size,tumor thrombus or liver cirrhosis. The expression level of p33 ^ING1b mRNA in tumors With positive p53 expression （0. 492 ±0. 196） was signficanfly higher than that with negative p53 expression （0.363± 0. 176） （ P 〈 0.05 ）. Conclusion Down - reg- ulated expression of p33^ING1b may play an important role in the tumorigenesis and development of PHC. ^ING1b might be an useful marker in evaluating tumor differentiation ,invasion and prognosis. Abnormal expression of ^ING1b gene may be one of the mechanisms of p53 dysfunction in HCC.