肝癌HIF-1α表达与多排CT血供分型的相关性研究与临床意义

Hepatocellular carcinoma: a correlative study between its blood supply types on multislice spiral CT and HIF-1α expression

目的:研究肝癌多排CT血供分型与缺氧诱导因子-1α(HIF-1α)表达水平的相关性。材料和方法:对34例手术病理证实的肝癌术前行多排螺旋CT双期扫描观察血供分型,免疫组化SP法检测肝癌Edmondson-Steiner分级对应的HIF-1α和VEGF表达水平,将两者进行对比分析。结果:肝动脉供血型中HIF-1α表达强阳性和阳性最高,其次为肝动脉和门静脉双重供血型者。门静脉供血型和少血供型表达水平最低(P<0.001)。随Edmondson-Steiner分级增高,HIF-1α表达增高(P<0.05)。结论:多排CT双期扫描能够反映肝癌血供分型和血管生成,一定程度上推测HIF-1α表达水平和组织学分级,有利于包括抗血管生成治疗肿瘤方案的选择及预后评估。

Objective： To study the correlation between blood supply types on multislice spiral CT and hypoxia inducible factor-1α （HIF-1α） expression in hepatocellular carcinoma （HCC）. Methods： In 34 cases HCC identified with operation and pathology, the relationship between blood supply types on muhislice spiral CT during dual-phase scanning and the expression levels of HIF-1α and vascular endothelial growth factor （VEGF） in HCC with immunohistochemistry staining in SP and Edmondson-Steiner grading. Results： Among the four blood supply types, both the strong positive staining and the positive staining are the highest in the arterial blood supply type, followed by the arterial combining with portal blood supply type. The two lowest types are portal blood supply type and poorly blood supply type （P〈0.001）. With the increasing of the Edmondson- Steiner histological grades, the expression of HIF-1α levels also increase correspondingly（P〈0.05）. Conclusion： The blood supply type of HCC and the HIF-1α expression levels that reflects the HCC angiogenesis condition and the histological grades can be judged to some extent by using multislice spiral CT during dual-phase scanning. Hence it will be useful for the selecting of HCC treatment plans including anti-angiogenesis and evaluating the prognosis.