蛋白质／多肽的微流控二维芯片电泳

Protein/Peptides Electrophoresis Separation by Two-Dimensional Microfluidic Chip

在微流控芯片上构建多维分离系统,为蛋白质组学研究提供了一个有发展前景的高效分离分析技术平台。本文介绍了二维芯片电泳系统耦联模式的选取及正交性评价的方法;综述了针对蛋白质/多肽分离分析的各种耦联模式微流控二维芯片电泳分析系统,如胶束电动力学色谱(MEKC)与毛细管区带电泳(CZE),开管电色谱(OCEC)与CZE,等电聚焦(IEF)与CZE,IEF与SDS毛细管凝胶电泳(CGE),SDS-CGE与MEKC等。特别对二维电泳芯片切换接口的类型进行了分类,探讨了用于微流控二维芯片电泳系统的检测技术,并展望了微流控二维电泳芯片在蛋白质组学研究中的应用前景和发展方向。

A powerful tool is presented in the proteome studies by multi-dimensional separation system constructed on the micofluidic chip platform. Approaches to estimate the orthogonality between selected separation modes are presented in this paper. Microfluidic two-dimensional chip electrophoresis system of various coupling modes, such as micellar electrokinetic chromatography （MEKC） coupled with capillary zone electrophoresis （CZE）, open-channel electro-chromatography （OCEC）-CZE, isoelectric focusing （IEF）-CZE, IEF-SDS-capillary gel electrophoresis （CGE）, SDS-CGE-MEKC, etc., designed for protein/peptides separation are summarized. Particularly, types of switching interface for two-dimensional electrophoresis microchip are classified and detecting techniques for two-dimensional microchip electrophoresis are discussed. Application prospects and developing trends of microfluidic two-dimensional electrophoresis chip in the proteome studies are also discussed.