生物合成葡萄糖基-L-抗坏血酸产酶条件及转化条件优化

The optimization of enzyme producing condition and transformation condition to prepare glucopyranosyl-L-ascorbid acid

采用单因素实验确立微生物发酵法产环糊精糖基转移酶培养基基本条件和工艺条件,利用此酶催化L-抗坏血酸和糖基供体β-环糊精合成葡萄糖基-L-抗坏血酸(AA-2G),并通过正交实验考察不同加酶量、反应温度、pH等因素,确立环糊精糖基转移酶合成产物最佳条件为:在浓度0.04 mol/L L-抗坏血酸,酶量20 ml,pH值7.0,温度50℃下反应15 h。

The culture medium component and culture condition for producing cyclodextrin clycosyltransferase by Bacillus alkalophilus 7-12 was primarily established by single sactor design. The enzyme was used to transform L-ascorbic acid to glucopyranosyl-L-ascorbid acid （AA-2G）. Its optimal transformation condition was established by srthogonal test design. The best condition is 0.04 mol/L Vc, the enzyme volume 20 ml, pH 7.0, reacting under 500℃ for 15 h.