鲍氏针层孔菌原生质体融合育种的研究

The Breeding of Phellinus baumii by Protoplast Fusion

目的:鲍氏针层孔菌优良菌株的选育。方法:通过5.8SrDNA-ITS序列,生长速度、液体发酵生产菌丝体和胞外多糖产量等方面,对不同来源的菌株进行分析比较,筛选出优良的亲本菌株。在此基础上,采用双灭活标记,原生质体PEG诱导融合的方法。结果:筛选出亲本菌株SA02和SA04,以及编号为R002、R003、R005等三个融合菌株与两亲本菌株都具有拮抗反应,且菌落性状表现出不同于亲本的性状。结论:R002、R003和R005初步确定为双亲株的融合子。

Objective： To breed good strains by protoplast fusion in Phellinus baumii. Methods： 5.8S rDNA-ITS sequences, mycelial growth rate, mycelial biomass and exopolysaccharides （EPS） production of 7 strains in different origins were analyzed, respectively. Then the inactivated parent protoplast and the PEG induction were used in the process of protoplast fusion. Results ： The strain P. baumii SA02 and P. baumii SA04 were selected as the parent strains. R002, R003 and R005 had antagonistic effects with parent strains. Conclusion ： R002, R003 and R005 are fusants of the parent strains.